1 Experiments

1. Chemostats cultures growing on Ethanol and limited on:
   1. Ethanol: [Ethanol] = 30mM
   2. Nitrogen: [N] = 1.5 mM
   3. Phosphate [P] = 0.15mM
2. Growth Rates
   1. Chemostat dilution rates: 0.05, 0.1, 0.14 per hour
   2. Corresponding to: 14, 7, 5 hours per doubling
3. Each chemostat culture was sampled 3 times, once every 48 hours
   1. mRNA. References for the micro-arrays: Glucose limited cultures growing at dilution rates 0.025 and 0.25 per hour
   2. Metabolites. Reference the metabolites: Two phosphate glucose limited culture growing at dilution rate 0.05 per hour

2 Determining Limiting Concentrations in Batch:

a) Ethanol Limitation

b) Nitrogen Limitation

c) Phosphate Limitation