Survival During Nutrient Starvation

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Figure S1. Comparison of absolute death rate estimates inferred from pooled experiment for uptags (circles) and downtags (triangles) with individual mutant analysis in pure cultures (crosses).
Data are presented for ATG10Δ0 starved for (A) phosphate and (B) leucine and PEX13%G∆%@0 starved for (C) phosphate and (D) leucine. Barcode counts are the normalized as described in (methods). Pure culture counts are number of viable cells/L. supplementary figures and tables 

Table S1. Summary of sequencing experiments.
All sequencing data that passed filters in the Illumina software pipeline were analyzed and processed with custom Perl scripts.

Table S2. Modeled relative survival rates for all mutants starved for phosphate.
We performed regression analysis for 6,806 barcodes corresponding to 4,337 unique genes. We determined that 1,333 genes behave significantly differently than the overall population at an FDR of 5%.

Table S3. Modeled relative survival rates for all mutants starved for leucine.
We performed regression analysis for 6,740 barcodes corresponding to 4299 unique genes. We determined that 3,951 genes behave significantly differently than the overall population at an FDR of 5%.

Table S4. Modeled absolute survival rates for all mutants starved for phosphate.
We performed regression analysis for 6,806 barcodes corresponding to 4,337 unique genes. We calculated significant absolute death rates for 4,143 genes at an FDR of 5%.

Table S5. Modeled absolute survival rates for all mutants starved for leucine.
We performed regression analysis for 6,730 barcodes corresponding to 4,239 unique genes. We calculated significant absolute death rates for 3,591 genes at an FDR of 5%.

Table S6. Complete results for gene class enrichment analysis.
We tested gene classes defined by GO terms, GO slim terms, protein complexes, biochemical pathways, high throughput phenotypic studies and gene expression studies for significant non-random distributions of half lives.


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