TABLE S1
Summary of all barcode
sequencing experiments
|
Expt.
|
Experiment Description
|
Figure
|
Total Reads
|
Uptags
|
Dntags
|
Unmapped
|
|
1
|
Single
barcode
|
Figure 2A
|
2,340,984
|
2,340,984
|
n/a
|
n/a
|
|
2
|
VB_s_1
|
Figure 2C
|
4,277,717
|
2,124459
(49.7%)
|
2,153,258
(50.3%)
|
1,155,131
(27.0%)
|
|
3
|
PCR 30 cycles
|
Figure 2B
|
3,585,742
|
552,455
(15.4%)
|
3,033,287
(84.6%)
|
998,506
(27.8%)
|
|
4
|
PCR 25 cycles
|
Figure 2B
|
7,737,553
|
3,477,644
(44.9%)
|
4,259,909
(55.0%)
|
1,970,333
(25.5%)
|
|
5
|
PCR 20 cycles
|
Figure 2B
|
3,659,194
|
1,532,589
(41.9%)
|
2,126,605
(58.1%)
|
900,199
(24.6%)
|
|
6
|
PCR 15 cycles
|
Figure 2B
|
1,029,015
|
657,650
(63.9%)
|
371,365 (36.21%)
|
253,044
(24.6%)
|
|
7
|
VB_s_1_repeat
|
Figure 2C
|
7,594,300
|
2,793,694
(36.8%)
|
4,800,606
(63.2%)
|
1,908,887
(25.1%)
|
|
8
|
Unselected
pool 1
|
Figures 2D, 4 and 5
|
8,185,598
|
2,134,658 (26.1%)
|
6,050,940 (73.9%)
|
1935672
(23.6%)
|
|
9
|
Unselected
pool 2
|
Figures 2D, 4 and 5
|
7,457,342
|
1,431,691 (19.2%)
|
6,025,651 (80.8%)
|
1786893
(24.0%)
|
|
10
|
Phosphate
starvation t = 0hr
|
Figures 4
and 5
|
7,360,688
|
1,821,104 (24.7%)
|
5,539,584 (75.2%)
|
2,044,378
(27.8%)
|
|
11
|
Phosphate
starvation t = 24hr
|
Figures 4 and 5
|
7,969,303
|
2,463,639 (30.9%)
|
5,505,664 (69.1%)
|
2,172,916
(27.3%)
|
|
12
|
Phosphate
starvation t = 48hr
|
Figures 4 and 5
|
7,740,944
|
2,383,718 (30.8%)
|
5,357,226 (69.3%)
|
1,904,457
(24.6%)
|
|
13
|
Phosphate
starvation t = 72hr
|
Figures 4 and 5
|
6,201,163
|
2,114,157 (34.1%)
|
4,087,006 (65.9%)
|
1,509,900
(24.3%)
|
|
14
|
Phosphate
starvation t = 99hr
|
Figures 4 and 5
|
6,552,758
|
1,321,294 (20.2%)
|
5,231,464 (79.8%)
|
1,623,590
(24.8%)
|
|
15
|
Phosphate
starvation t = 144hr
|
Figures 4 and 5
|
8,331,886
|
3,714,888 (44.6%)
|
4,616,998 (55.4%)
|
2,030,725
(24.4%)
|
|
16
|
Phosphate
starvation t = 267hr
|
Figures 4 and 5
|
8,977,470
|
1,555,925 (17.3%)
|
7,421,545 (82.7%)
|
2,531,095
(28.2%)
|
|
17
|
Phosphate
starvation t = 473hr
|
Figures 4 and 5
|
4,815,151
|
1,029,621 (21.4%)
|
3,785,530 (78.6%)
|
1,165,598
(24.2%)
|
|
18
|
Leucine
starvation t = 0hr
|
Figures 4 and 5
|
8,367,434
|
2,347,434 (28.0%)
|
6,020,000 (71.9%)
|
2,041,278
(24.4%)
|
|
19
|
Leucine
starvation t = 24hr
|
Figures 4 and 5
|
8,822,841
|
2,330,193 (26.4%)
|
6,492,648 (73.6%)
|
2,155,783
(24.4%)
|
|
20
|
Leucine
starvation t = 48hr
|
Figures 4 and 5
|
9,494,929
|
3,845,443 (40.5%)
|
5,649,486 (59.5%)
|
2,342,985
(24.7%)
|
|
21
|
Leucine
starvation t = 72hr
|
Figures 4 and 5
|
7,512,308
|
2,243,459 (29.9%)
|
5,268,849 (70.1%)
|
1,853,548
(24.7%)
|
|
22
|
Leucine
starvation t = 99hr
|
Figures 4 and 5
|
8,884,437
|
2,217,260 (25.0%)
|
6,667,177 (75.0%)
|
2,215,985
(24.9%)
|
|
23
|
Leucine
starvation t = 144hr
|
Figures 4 and 5
|
8,383,630
|
2,486,648 (29.7%)
|
5,896,982 (70.3%)
|
2,107,793
(25.1%)
|
|
24
|
Leucine
starvation t = 267hr
|
Figures 4 and 5
|
8,711,905
|
1,579,689 (18.1%)
|
7,132,216 (81.9%)
|
2,427,867
(27.9%)
|
|
25
|
Leucine starvation
t = 473hr
|
Figures 4 and 5
|
8,644,145
|
1,779,999 (20.6%)
|
6,864,146 (79.4%)
|
2,938,915
(34.0%)
|
|