Chemostat Experiments

1. Chemostats cultures of auxotrophs growing on glucose 20g/L and limited on:
   1. his3 auxotroph (DBY9496) limited on Histidine: [His] = 4mg/L.
      • View his biosynthetic pathway.
   2. lys2 auxotroph (DBY919) limited on Lysine: [Lys] = 6mg/L.
      • View lys biosynthetic pathway.
   3. ura3 auxotroph (DBY9492) limited by Uracil: [Ura] = 5mg/L.
      • View reaction pathway.
2. Growth Rates
   1. Chemostat dilution rates: 0.05, 0.10, 0.15, 0.2, 0.25, 0.30 per hour
   2. Corresponding to: 13.8, 6.9, 4.6, 3.5, 2.8, 2.3 hours per doubling
3. Measurements
   1. mRNA. Reference for the microaarays: Glucose limited culture (DBY10085) growing at dilution rate 0.25 per hour
   2. Measure residual [Glucose] & [Ethanol] and cell sizes.