- Chemostats cultures of auxotrophs growing on glucose 20g/L and limited on:
auxotroph (DBY9496) limited on Histidine: [His] = 4mg/L.
auxotroph (DBY919) limited on Lysine: [Lys] = 6mg/L.
- ura3 auxotroph (DBY9492) limited by Uracil: [Ura] = 5mg/L.
- Growth Rates
- Chemostat dilution rates: 0.05, 0.10, 0.15, 0.2, 0.25, 0.30 per hour
- Corresponding to: 13.8, 6.9, 4.6, 3.5, 2.8, 2.3 hours per doubling
- mRNA. Reference for the microaarays: Glucose limited culture (DBY10085) growing at dilution rate 0.25 per hour
- Measure residual [Glucose] & [Ethanol] and cell sizes.